Showing the last 30 topics from:

TopicRepliesViewsActivity
High but variable sequence loss (32-68%) during DADA2 filtering with V3-V4 amplicons (MiSeq 600 cycles)235Thu Apr 09 2026
Problem with cache using qiime cutadapt1057Mon Mar 30 2026
Possible bug in qiime cutadapt demux paired (2024.10)675Wed Jan 28 2026
Q2-cutadapt add "--nextseq-trim" option373Fri Feb 27 2026
Huge difference of DADA2 output with and without removing primers586Tue Jan 27 2026
qiime cutadapt does not have an untrimmed sequences output5117Mon Dec 01 2025
ITS denoising truncation260Wed Oct 15 2025
Duplicate barcode error, but barcodes listed are not the same.791Tue Aug 19 2025
Inconsistent results with discard untrimmed reads in cutadapt7120Thu Jul 10 2025
cutadapt outside of QIIME2156Mon Aug 04 2025
QIIME 2 Meta-Analysis of Rhizosphere Microbiome with Mixed V3-V4/V4 Data: Best Practices for Downstream Analysis14472Thu Apr 17 2025
Analysing V4/V5 16S rRNA illumina Miseq paired-end reads.16371Tue Mar 18 2025
Trimming Paired-End Sequences with cutadapt While Maintaining Pairing Integrity274Mon Feb 10 2025
Which position is best for trimming in a paired-end approach?11143Fri Jan 24 2025
Comparing amplicon studies that use different primers9180Wed Nov 20 2024
Trimming 5bp in QIIME2 2024.5 Amplicon441Thu Nov 14 2024
Error messages after cutadapt, but got trimmed files.5131Thu Nov 07 2024
Cut adapt results in empty files4113Fri Oct 18 2024
QIIME2 Demultiplexing Sequence7202Tue Sep 03 2024
One vs two adaptor sequences using cutadapt499Fri Sep 27 2024
V3-V4 16s - variable size after merging7238Mon Aug 05 2024
problem with cutadapt trim-single6137Tue Jul 02 2024
Error during deblur process8302Sat May 11 2024
Cutadapt - trimming adaptor and primer sequences3225Sat May 11 2024
Missing plugins to load Artifact to run cut adapt2142Tue May 07 2024
Seeking Advice on Primer and Adapter Trimming for MiSeq Amplicon Sequencing Data Using Cutadapt0169Thu Jun 13 2024
Removal of Illumina Adapters1193Thu May 30 2024
Primer show up again after removal using cutadapt in qiime2 DADA211404Mon May 06 2024
I fell into the swamp of 273bp after trimming adapters and primers through cutadapt...8241Mon Mar 18 2024
Pyrosequence work in Qiime2 (Barcode)11277Fri Mar 15 2024