Showing the last 30 topics from:

TopicRepliesViewsActivity
16S CCS without primers: how to skip --p-front/--p-adapter in denoise-ccs?312Tue Aug 26 2025
Percentage of reads discarded after running DADA2 on NextSeq data117Mon Aug 25 2025
Effect of primer removal on chimera detection in DADA21089Mon Aug 25 2025
Less percentage of reads merged in qiime-DADA2 even with enough overlapping length present.754Thu Jul 10 2025
dada2 error: rcpp/SparseArray220Wed Jul 09 2025
Shall I need to remove ASV with prevelance in one sample?433Fri Aug 08 2025
Why is there a size difference between my PCR product (~400 bp) and the final representative sequence (313 bp)?428Tue Jul 22 2025
Please help interpreting demux data240Tue Jun 17 2025
Minimum length of read in ASV (DADA2)676Sun Jun 08 2025
PICRUST2 Input Data setting132Tue Jul 08 2025
Understanding min sequence length of F and R/ quality scores and how it is impacting dada2 downstream15109Tue Jun 03 2025
Denoise pair returning DataFrame' object has no attribute 'map' error651Mon Jun 02 2025
dada2-paired giving return code 1741Tue May 27 2025
DADA2 fails silently on SLURM cluster (QIIME2 2024.10) — No .qza outputs, no error messages220Thu May 22 2025
Error with DADA2 with sequences of different runs.452Wed May 21 2025
Low percentage of non-quimeric reads after dada2444Wed May 14 2025
Shotgun sequencing and Trimming225Tue Jun 03 2025
Losing a lot of reads during merging480Fri Apr 18 2025
Can trimming and dada 2344Mon May 19 2025
QIIME 2 Meta-Analysis of Rhizosphere Microbiome with Mixed V3-V4/V4 Data: Best Practices for Downstream Analysis14302Thu Apr 17 2025
Questions about the running time of qiime dada2 denoise-ccs459Fri Apr 11 2025
trunc length setting in dada2213Fri Apr 11 2025
dada2 failed while running nextseq data13182Thu Apr 10 2025
low proportion of identified ITS - OTU features363Fri Mar 28 2025
dada2 denoise-ccs most reads were filtered out10104Tue Mar 25 2025
Alpha diversity with filtered raw data or unfiltered raw data162Thu Apr 24 2025
How can I perform learnErrors on a subset of reads?338Mon Apr 07 2025
alpha and beta diversity244Sun Apr 06 2025
Why do we get higher species level assignment with an older GTDB classifier than with a more recent one?8136Thu Mar 27 2025
Expose more options for DADA2 mergePairs function159Tue Mar 18 2025