| Observed ASV richness drops after adding 3rd MiSeq library β why do I lose ~100 species when I merge libraries? | | 4 | 23 | Thu Oct 16 2025 |
| ITS denoising truncation | | 2 | 27 | Wed Oct 15 2025 |
| Need help choosing truncation lengths for DADA2 and interpreting the demultiplexed sequence count summary. | | 2 | 30 | Mon Oct 13 2025 |
| QIIME2 non-chimeric reads | | 3 | 33 | Mon Nov 10 2025 |
| DADA2 -- denoise | | 2 | 41 | Fri Nov 07 2025 |
| Significant 16S sequence loss while merging w/ DADA2, various truncation settings used | | 2 | 56 | Wed Nov 05 2025 |
| DADA2 Fails on AVITI Data | | 7 | 68 | Wed Oct 29 2025 |
| qiime dada2 denoise-paired is unresponsive | | 2 | 34 | Mon Oct 27 2025 |
| Interpreting KEGG vs MetaCyc pathways in 16S (PICRUSt2) functional prediction | | 1 | 29 | Sun Oct 19 2025 |
| Trained classifier output not found | | 7 | 67 | Thu Sep 11 2025 |
| Converting 16S V3βV4 ASV reads to absolute copy numbers (qPCR / spike-in calibration?) | | 3 | 65 | Thu Oct 09 2025 |
| Chimera removal dilemma in 16S V3βV4: necessary when using Kraken2/Bracken? | | 8 | 76 | Fri Sep 26 2025 |
| 16S CCS without primers: how to skip --p-front/--p-adapter in denoise-ccs? | | 4 | 57 | Tue Aug 26 2025 |
| Effect of primer removal on chimera detection in DADA2 | | 11 | 148 | Mon Aug 25 2025 |
| Why is non-chimera read count often referred to as sequencing depth in 16S rRNA analysis? | | 4 | 90 | Thu Sep 18 2025 |
| Percentage of reads discarded after running DADA2 on NextSeq data | | 1 | 47 | Mon Aug 25 2025 |
| Less percentage of reads merged in qiime-DADA2 even with enough overlapping length present. | | 7 | 66 | Thu Jul 10 2025 |
| dada2 error: rcpp/SparseArray | | 2 | 31 | Wed Jul 09 2025 |
| Shall I need to remove ASV with prevelance in one sample? | | 4 | 52 | Fri Aug 08 2025 |
| Why is there a size difference between my PCR product (~400 bp) and the final representative sequence (313 bp)? | | 4 | 67 | Tue Jul 22 2025 |
| Please help interpreting demux data | | 2 | 56 | Tue Jun 17 2025 |
| Minimum length of read in ASV (DADA2) | | 6 | 100 | Sun Jun 08 2025 |
| PICRUST2 Input Data setting | | 1 | 52 | Tue Jul 08 2025 |
| Understanding min sequence length of F and R/ quality scores and how it is impacting dada2 downstream | | 15 | 128 | Tue Jun 03 2025 |
| Denoise pair returning DataFrame' object has no attribute 'map' error | | 6 | 66 | Mon Jun 02 2025 |
| dada2-paired giving return code 1 | | 7 | 54 | Tue May 27 2025 |
| DADA2 fails silently on SLURM cluster (QIIME2 2024.10) β No .qza outputs, no error messages | | 2 | 31 | Thu May 22 2025 |
| Error with DADA2 with sequences of different runs. | | 4 | 59 | Wed May 21 2025 |
| Shotgun sequencing and Trimming | | 2 | 36 | Tue Jun 03 2025 |
| Can trimming and dada 2 | | 3 | 52 | Mon May 19 2025 |
