| Converting 16S V3–V4 ASV reads to absolute copy numbers (qPCR / spike-in calibration?) | | 1 | 13 | Sun Oct 05 2025 |
| Chimera removal dilemma in 16S V3–V4: necessary when using Kraken2/Bracken? | | 8 | 58 | Fri Sep 26 2025 |
| 16S CCS without primers: how to skip --p-front/--p-adapter in denoise-ccs? | | 4 | 37 | Tue Aug 26 2025 |
| Effect of primer removal on chimera detection in DADA2 | | 11 | 129 | Mon Aug 25 2025 |
| Why is non-chimera read count often referred to as sequencing depth in 16S rRNA analysis? | | 4 | 72 | Thu Sep 18 2025 |
| Trained classifier output not found | | 6 | 47 | Thu Sep 11 2025 |
| Percentage of reads discarded after running DADA2 on NextSeq data | | 1 | 39 | Mon Aug 25 2025 |
| Less percentage of reads merged in qiime-DADA2 even with enough overlapping length present. | | 7 | 58 | Thu Jul 10 2025 |
| dada2 error: rcpp/SparseArray | | 2 | 22 | Wed Jul 09 2025 |
| Shall I need to remove ASV with prevelance in one sample? | | 4 | 38 | Fri Aug 08 2025 |
| Why is there a size difference between my PCR product (~400 bp) and the final representative sequence (313 bp)? | | 4 | 46 | Tue Jul 22 2025 |
| Please help interpreting demux data | | 2 | 41 | Tue Jun 17 2025 |
| Minimum length of read in ASV (DADA2) | | 6 | 84 | Sun Jun 08 2025 |
| PICRUST2 Input Data setting | | 1 | 39 | Tue Jul 08 2025 |
| Understanding min sequence length of F and R/ quality scores and how it is impacting dada2 downstream | | 15 | 114 | Tue Jun 03 2025 |
| Denoise pair returning DataFrame' object has no attribute 'map' error | | 6 | 56 | Mon Jun 02 2025 |
| dada2-paired giving return code 1 | | 7 | 42 | Tue May 27 2025 |
| DADA2 fails silently on SLURM cluster (QIIME2 2024.10) — No .qza outputs, no error messages | | 2 | 21 | Thu May 22 2025 |
| Error with DADA2 with sequences of different runs. | | 4 | 52 | Wed May 21 2025 |
| Low percentage of non-quimeric reads after dada2 | | 4 | 47 | Wed May 14 2025 |
| Shotgun sequencing and Trimming | | 2 | 26 | Tue Jun 03 2025 |
| Losing a lot of reads during merging | | 4 | 81 | Fri Apr 18 2025 |
| Can trimming and dada 2 | | 3 | 48 | Mon May 19 2025 |
| QIIME 2 Meta-Analysis of Rhizosphere Microbiome with Mixed V3-V4/V4 Data: Best Practices for Downstream Analysis | | 14 | 340 | Thu Apr 17 2025 |
| Questions about the running time of qiime dada2 denoise-ccs | | 4 | 70 | Fri Apr 11 2025 |
| trunc length setting in dada2 | | 2 | 14 | Fri Apr 11 2025 |
| dada2 failed while running nextseq data | | 13 | 202 | Thu Apr 10 2025 |
| Alpha diversity with filtered raw data or unfiltered raw data | | 1 | 65 | Thu Apr 24 2025 |
| How can I perform learnErrors on a subset of reads? | | 3 | 38 | Mon Apr 07 2025 |
| alpha and beta diversity | | 2 | 48 | Sun Apr 06 2025 |
